节节麦不同生育期叶片蜡质组成和超微形态的变化

为了解节节麦(Aegilops tauschii Coss.)不同生育期叶片表皮蜡质组成及晶体形态的变化,对节节麦叶片蜡质进行提取,利用气相色谱-质谱(GC-MS)联用仪和气相色谱-火焰离子化检测仪(GC-FID)对蜡质进行了定性和定量分析,并采用扫描电镜(SEM)对叶片表面蜡质晶体形态进行了观察。结果表明,节节麦叶表皮蜡质经GC-MS分析,共鉴定出化合物21种,主要以初级醇为主(苗期、抽穗期和灌浆期相对含量分别为85%、84%和70%),并含有少量的烷烃、醛、酮和脂肪酸;随着节节麦的生长发育,蜡质总量不断积累,烷烃含量极显著地升高,其中以C29烷烃的增加最明显,而初级醇含量显著降低,其中C26醇的相对含量降低最明显(苗期、抽穗期和灌浆期相对含量分别为80%、76%和63%),其他各类化合物组分变化不大。经扫描电镜观察,节节麦叶片近轴和远轴面的蜡质晶体形态无明显差别,苗期均为片状,抽穗期大部分仍以片状形态存在,并且蜡质晶体的密集程度比苗期稀疏,灌浆期蜡质晶体的密集程度变得更加稀疏。这些变化可能与抽穗期、灌浆期烷烃含量的增加和初级醇含量的减少有关。     

初探酵素生物菌肥料对盐碱地水稻生长的影响

探讨了大安市盐碱地施酵素生物菌肥料，采用简塑盘大棚育壮秧，浅翻（ 耙） ，洗盐排碱，配施氮、磷、钾、锌肥，综合防治病虫草害等综合栽培措施，水稻公顷产量获得８ ５００ ｋｇ 以上的丰产技术．其产量结构为每平方米穴数为２５ 穴，每平方米穗数６００ 穗，穗总粒数为８７ 粒，结实率为８９ ％ ，千粒重为２７ ．７ ｋｇ ，产量为８ ５００ ｋｇ．     

我国体育经纪组织发展困境及破解路径分析

以文献研究、逻辑分析为主要研究方法，分析了当前我国体育经纪组织发展所面临的困境，指出了我国体育经纪组织品牌化发展的必要性。从五个方面提出了我国体育经纪组织品牌化发展的思路：增强体育经纪组织品牌意识、实施体育经纪组织品牌战略、设计体育经纪组织品牌要素、塑造体育经纪组织品牌文化、打造体育经纪组织知名品牌。     

某增压器的整机动不平衡量偏大的工艺改进

通过对某四缸柴油机用增压器的整机动平衡通过率低的原因分析,找到影响整机动平衡的主要原因,通过工艺参数的调整,使整机动平衡达到设计要求.     

Repression of SFRP5 promoter activity by hepatitis B virus X protein

AIM: To investigate the molecular mechanism that hepatitis B virus X protein (HBx) inhibits the promoter activity of secreted frizzled-related protein 5 (SFRP5). METHODS: Serial truncated fragments of SFRP5 promoter were amplified and cloned into luciferase reporter vector pGL3-Basic. LO2 cells were transfected with recombinants, and then infected with the adenoviral vector expressing HBx protein (Ad-HBx) or the analogous adenovirus expressing green fluorescent protein (Ad-GFP) for control. The infection efficiency was examined under a fluorescence microscope and the activity of SFRP5 promoter was measured by luciferase assay. RESULTS: Truncated fragments of SFRP5 promoter were successfully constructed. Compared with the pGL3-Basic control, the luciferase activity was 6.32±0.04 of the -478 bp~+47 bp fragment, 5.79±0.32 of the -811 bp~+47 bp fragment, 3.59±0.34 of the -1 235 bp~+47 bp fragment, 3.86±0.39 of the -1 677 bp~+47 bp fragment and 3.26±0.42 of the -2 072 bp~+47 bp fragment, respectively. Overexpression of HBx inhibited the activity of SFRP5 promoter. The average inhibitory rates were 44% for -478 bp~+47b, 46% for -811 bp~+47 bp, 28% for -1 235 bp~+47 bp, 24% for -1 677 bp~+47 bp and 40% for -2 072 bp~+47 bp, respectively. CONCLUSION: HBx suppresses the activity of SFRP5 promoter, leading to down-regulation of SFRP5 expression.     

云计算在中小企业信息化中的应用研究

云计算是继网格计算后又一项正在兴起中的技术,它的出现使"互联网上众多的计算机成为一台虚拟超级计算机"的梦想逐渐变成了现实。概述了云计算的概念与原理,阐明了云计算在中小企业中实施的技术与优势,提出了云计算在中小企业实施中存在的问题以及解决问题的策略。     

信阳市苗木生产中存在的问题与对策

信阳市苗木生产发展迅速,但仍存在着品种单一、结构不合理、缺少品牌优势、科技含量低等问题。应采取统一规划、合理种植、因地制宜等技术措施,促进信阳市苗木生产的可持续发展。     

关于温州市推行水稻机械栽插的思考

文章首先分析了浙江省温州市机插的现状,指出加快突破插秧机械化技术瓶颈,已成为推进水稻生产全程机械化,实现农业现代化的重要主攻方向,要制订切实可行的水稻插秧机械化推进计划;从节省秧田、节水肥药、节本省工、高产稳产、避灾增效、五个方面阐述了机插的优势,从技术上可行、政策上有利、推广有依托、多数农民观念转变四个方面阐述了机插有利条件;最后提出推进水稻机插的对策与措施。     

单边支重轮半体切边-冲孔复合模设计

介绍了在曲柄压力机上切边冲孔的复合模结构设计、工作原理及装配和调试要求等.     

Design Method of Moment-strengthening on Tension Side When Load Un removed

Based on the linear strain distribution assumption, strain development of tensile bars and ultimate moment capacity of strengthening section, which strengthened on tension side with service load un removed, is investigated. It is showed that the higher the service load bending moment to original moment capacity ratio M 1k /M u1 is, the smaller the tensile strain of strengthening re barsis, and the more the amount of strengthening re barsis, the smaller the tensile strain of original re barsis. It also showed that,When original re bar area is relatively small and strengthening re bar area is adequate, both original and strengthening re bars may get yielded and a great increment in section moment capacity is achieved, even though the ration M 1k M u1 has a relatively high value.